Swiss Webster mouse splenocytes were unstimulated (left) or stimulated overnight with Con A (Product # 00-4978-03) (right). Cells were then stained with CD25 Monoclonal Antibody, eFluor 450 (Product # 48-0251-82) and 0.25 µg of CD69 Monoclonal Antibody, Brilliant Ultra Violet 496. Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Product # 00-6993-50).